TABLE 3.

Average density heights for Cα positions (sumf, as defined in Table 2) upon fitting the individual domains from the CHIKV E1E2 heterodimer and the Fab structures into the cryo-EM map at four quasiequivalent positions^a

Complex and domain	Avg density height upon:
	Independent domain fitting					T=4 fitting
	#1	#2	#3	#4	Avg	#1	#2	#3	#4	Avg
8B10 complex at pH 7.6
E1 I	35.7	34.9	35.2	34.5	35.1	34.4	35.1	34.0	34.2	34.4
E1 II	36.9	35.3	35.1	35.0	35.6	35.9	35.6	34.8	34.2	35.1
E1 III	34.9	35.7	35.1	34.7	35.1	34.4	35.2	35.0	34.8	34.9
E2 A	37.4	38.1	37.2	37.1	37.5	36.8	37.3	36.6	36.3	36.8
E2 B	29.7	28.9	28.4	28.0	28.8	28.2	27.9	28.5	27.2	28.0
E2 C	36.0	36.7	37.1	35.9	36.4	35.7	36.0	35.9	35.3	35.7
Fab V	31.1	30.5	30.7	30.9	30.8	30.7	29.3	29.1	29.5	29.7
Fab C	30.2	31.5	29.9	29.6	30.3	29.5	29.1	28.7	28.1	28.9
5F10 complex at pH 7.6
E1 I	36.2	37.1	37.4	36.0	36.7	35.7	35.9	34.8	34.5	35.2
E1 II	35.9	36.3	35.2	34.9	35.6	35.1	34.7	35.8	34.3	35.0
E1 III	35.5	35.2	34.9	34.3	35.0	35.6	35.1	35.0	34.7	35.1
E2 A	34.6	34.0	33.9	33.7	34.1	33.4	33.0	32.9	32.3	32.9
E2 B	37.3	37.1	36.8	36.2	36.8	36.6	37.1	36.6	35.8	36.5
E2 C	35.9	35.5	35.7	35.1	35.6	34.5	34.9	33.8	33.4	34.2
Fab V	28.1	27.3	27.9	27.1	27.6	28.0	28.3	27.6	27.2	27.8
Fab C	27.0	27.7	27.0	26.9	27.2	26.7	27.0	26.1	26.0	26.5
5F10 complex at pH 6.0
E1 I	37.1	36.2	36.9	36.4	36.7	36.3	35.7	35.2	35.0	35.6
E1 II	35.2	35.7	35.2	34.0	35.0	34.5	34.8	34.1	34.1	34.4
E1 III	35.3	36.1	35.0	34.9	35.3	35.5	35.0	34.7	34.4	34.9
E2 A	35.6	34.8	34.8	34.2	34.9	36.0	36.2	36.9	35.7	36.2
E2 B	38.0	38.3	37.2	37.0	37.6	37.9	37.4	38.2	37.0	37.6
E2 C	35.4	35.3	35.1	35.0	35.2	34.1	34.9	33.8	34.3	34.3
Fab V	26.2	26.9	25.4	25.2	26.0	29.7	30.5	30.2	28.7	29.8
Fab C	26.0	25.8	26.1	25.6	25.9	28.7	30.9	31.6	29.4	30.2

^aFab V, Fab variable; Fab C, Fab constant.