FIG 1.

Effect of low cholesterol concentrations on ASFV infection. (A) ASFV infectious cycle milestones. (B) Intracellular cholesterol before (Ctrl) and after incubation of cells in cholesterol-depleted medium (CHDM). (C) Graphical representation of viral p30 protein expression at 6 hpi and of viral replication at 16 hpi in ASFV-infected cells incubated with CHDM added at the indicated times. (D) Viral replication in cells shifted at 2 hpi to cholesterol-enriched medium at the indicated cholesterol concentration (100 or 500 μM) or DMEM with 2% fetal calf serum (not lipoprotein depleted). (E) Variations in ASFV, VV, and Adv infectivity in Vero cells incubated in control medium or CHDM. Infected cells were detected by fluorescence-activated cell sorter (FACS) analysis and percentages normalized to controls. Statistically significant differences are indicated by asterisks (***, P < 0.001; **, P < 0.01). Representative FACS profiles of control, ASFV-infected, or ASFV-infected cells in CHDM are shown.