Increased uptake and free cholesterol pools upon ASFV infection. (A) Quantitation of total (dark bars), free (gray bars), and esterified (white bars) cholesterol pools in mock- or ASFV-infected cells at several time points. Values were normalized to mock-infected control values at each time point. (B) Bodipy-cholesterol uptake after ASFV infection at 1 and 3 hpi compared to that in control cells in the graph. representative images are shown. **, P < 0.01; *, P < 0.05. Bar, 10 μm.