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Alterations of cholesterol distribution and endosomal traffic with U18666A. Ctrl, cholesterol distribution in control cells with normal cholesterol efflux. CD63 and LBPA are stained in green and cholesterol in red. Upon U18666A treatment, inhibition of cholesterol efflux from endosomes caused cholesterol-laden vesicular clustering at the perinuclear area, while free cholesterol was reduced in other cellular locations such as the plasma membrane. Bar, 10 μm.
