FIG 5.

A cholesterol efflux block impairs viral exit from endosomes. TEM of control or ASFV-infected cells at 45 mpi is shown. DMSO-treated control cells with remnants of viral cores (arrowheads) are found in endosomes with intraluminal vesicles (arrow). In BAF-treated cells, virions with intact capsids were found in endosomes (arrowhead), as BAF-induced endosomal acidification inhibition impairs virus decapsidation. Scale bars, 500 nm. Bottom panels, TEM images of U18666A-treated and ASFV-infected cells with vesicular aggregates and “onion-leaf” images characteristic of lipids at 45 mpi. Virions are shown inside vesicles close to the nucleus (arrowheads).Scale bars, 500 nm. N, nucleus.