Skip to main content
. 2015 Mar 12;48(1):334–344. doi: 10.4143/crt.2014.311

Fig. 4.

Fig. 4.

Zinc finger protein 488 (ZNF488) activates the WNT/β-catenin pathway to induce the epithelial mesenchymal transition (EMT) transcription factors Tcf/Lef which is capable of triggering EMT. (A) The protein level of E-cadherin, α-catenin, vimentin, and N-cadherin were detected by western blotting analysis. (B) Immunofluorescence staining to dectect the changes of E-cadherin, ZO-1, vimentin, and N-cadherin. (C) Luciferase reporter assays using TopFlash/FopFlash reporter plasmids to monitor the activity of Tcf/Lef transcription factor. (D) Western blotting analysis to detect the expression of β-catenin, phospho-GSK3β (Ser9), GSK3β, Slug, and Snail. GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Data are presented as the mean±standard deviation, and p-values were calculated with Student’s t test.