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. 2016 Jan 11;11(1):e0146930. doi: 10.1371/journal.pone.0146930

Fig 1. SEMA7A is induced during lung injury.

Fig 1

Lungs from WT animals were harvested 4 hours after exposure to either NaCl or LPS inhalation. A) Relative mRNA of SEMA7A in lung tissue determined by qPCR following LPS inhalation (n≥5/group). B) Western blot analysis of SEMA7A (n = 3/group) and C) Immunofluorescence analysis of SEMA7A (green), cytokeratin as epithelial cell marker (red) in the lung following either NaCl or LPS inhalation, cell DNA marker DAPI (blue) and merge (yellow) (n = 4/group). D) Immunofluorescence analysis of SEMA7A (green), von Willebrand Factor (vWF) as endothelial cell marker (red) in the lung following either NaCl or LPS inhalation, cell DNA marker DAPI (blue) and merge (yellow) (n = 4/group). For negative controls see S1 Fig (all the data are expressed as the mean±SEM, *P < 0.05, as indicated).