Table 2. SPR Confirms High Affinity Binding and Increased On Rate.
| compound | KD (nM) | ka (1/M*s) | kd (1/s) |
|---|---|---|---|
| 1Za | 71 | (2.2 ± 0.8) × 104 | (1.4 ± 0.1) × 10–3 |
| 3Zb | 6.7 ± 3.7 | (3.2 ± 1.5) × 105 | (2.7 ± 1.8) × 10–3 |
| 4Eb | 13 ± 5.2 | (1.6 ± 0.7) × 105 | (1.9 ± 0.5) × 10–3 |
a
The values for compound 1Z were previously reported.19
b
SPR was carried out in 150 mM NaCl and 10 mM HEPES. Injections were repeated twice for consistency. Curves were fit individually to a mass transfer model, and the error reported is standard deviation of all fits.