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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Biochemistry. 2015 Dec 2;54(49):7248–7260. doi: 10.1021/acs.biochem.5b01082

Figure 2.

Figure 2

Nucleophilic activity of imidazole in the dephosphorylation of CheY-P. Phosphorimaging scans of TLC plate showing products of [32P]CheY-P dephosphorylation reactions (A) in the absence or presence of 250 mM imidazole at pH 7.5 and (B) in the presence of 250 mM imidazole at pH 4.9 (buffered with 250 mM sodium acetate) or pH 8.2 (buffered with 250 mM Tris). (C) Phosphorimaging scans of SDS-PAGE gels showing the loss of 32P from [32P]CheY-P in the presence of 250 mM imidazole at pH 4.9 (top panel) or pH 8.2 (bottom panel).