Figure 3.

Genetic deletion of the PGE₂ EP2 receptor reduces brain ferric iron Perls’ staining content after ICH. WT and EP2^−/− mice underwent ICH and were sacrificed at 72 hr for determination of ferric iron content by Perls’ staining of brain sections. (a) Representative high magnification images of coronal brain sections showing ferric iron accumulation (blue) in perihematomal regions from WT (left panel) and EP2^−/− mice (right panel). Square selections in the inserts denote magnified regions. (b) Quantification of blue positive pixel count in the ipsilateral hemisphere showed that EP2^−/− mice had significantly less ferric iron deposition (WT: n = 7, EP2^−/−: n = 10, *p < .05). No ferric iron was seen in the contralateral hemisphere for any of the mice in the study. PGE₂ = prostaglandin E₂; EP2 = E prostanoid receptor subtype 2; ICH = intracerebral hemorrhage; WT = wildtype.