Figure 6.

Genetic deletion of the PGE₂ EP2 receptor reduces peripheral neutrophil infiltration after ICH. Seventy-two hours after ICH, WT and EP2^−/− mice were sacrificed and brains processed for MPO immunohistochemistry to evaluate peripheral neutrophil infiltration. (a) Representative high magnification images of coronal brain sections showing diffusely distributed MPO + cells within the lesion. Square selections in the inserts denote magnified regions. (b) Quantification of brown positive pixel count in the ipsilateral hemisphere showed that EP2^−/− mice had significantly less peripheral neutrophil infiltration (WT: n = 5, EP2^−/−: n = 10, *p < .05). No MPO + cells were seen outside of the injured brain area for any of the mice in the study. PGE₂ = prostaglandin E₂; EP2 = E prostanoid receptor subtype 2; ICH = intracerebral hemorrhage; WT = wildtype; MPO = myeloperoxidase.