Figure 7.

Effect of PGE₂ EP2 receptor deletion on BBB breakdown after ICH. Seventy-two hours after ICH, WT and EP2^−/− mice were sacrificed and brains processed for IgG immunohistochemistry to evaluate BBB breakdown. (a) Representative images of coronal brain sections showing more diffuse disruption of the BBB in WT mice. Square boxes denote the location of high magnification inserts. (b) Quantification of whole brain brown positive pixel count showed that EP2^−/− mice strongly tended to have reduced BBB breakdown (WT: n = 6, EP2^−/−: n = 10, p = .0668). PGE₂ = prostaglandin E₂; EP2 = E prostanoid receptor subtype 2; ICH = intracerebral hemorrhage; WT = wildtype; BBB = blood–brain barrier; IgG = immunoglobulin G.