Fig. 1.

Osteolytica is a user-friendly interface that rapidly measures the number and area of osteolytic lesions. (A) Plain radiograph of a mouse tibia used as the original template for manual lesion counts. (B) Micro-CT 2-D reconstruction of a mouse tibiae showing the 3 aspects used as templates for 2-D ImageJ analysis to assess lesion area and number. (C) The 3 stages of Osteolytica, showing (i.) volume selection, (ii.) volume expansion and (iii.) detection and measurement of lesions. (D) Representative screenshots of the 3 steps of the Osteolytica software showing (i.) volume selection, where the user loads the dataset and selects the volume of interest, and the software removes any unattached floating volumes (ii.) maximum lesion diameter specification, where the user either manually enters the diameter or draws it on the screen and (iii.) lesion analysis results, where the software lists the area of every lesion measured, as well as total lesion area and total lesion proportion of the volume. Lesions can be deselected at this stage if required. (E) Flow diagram showing the 3 steps of Osteolytica and intermediate computer processes.