Figure 1.

Gene expression of Toll like Receptors, cytokines and antimicrobial peptides in corneal ulcers from patients with C. pseudodiphtheriticum keratitis. (A) Representative corneal ulcer of patient with C. pseudodiphtheriticum keratitis and Gram staining of corneal ulcer material showing Gram-positive C. pseudodiphtheriticum. RNA was isolated from corneal ulcers, reverse transcribed and quantitative PCR was done. Data points represent individual patients infected with C. pseudodiphtheriticum, and the values are presented either as log of relative expression or fold change in relation to uninfected donor corneas. The expressions of Toll like Receptors (B), proinflammatory cytokines (C) and antimicrobial peptides are (D) are shown. The representative gel images of the amplified products of antimicrobial peptides are shown (E). (**) designates P < 0.005.