Figure 2.

Expression and activation of Toll like Receptors by C. pseudodiphtheriticum in human corneal epithelial cells. Human corneal epithelial cells were infected with C. pseudodiphtheriticum for 4 hours, washed and RNA was isolated, reverse transcribed and quantitative PCR was done to determine the expression of Toll like Receptors in response to C. pseudodiphtheriticum (A); Western blot analysis of phosphorylation of IkBα in HCEC in response to C. pseudodiphtheriticum (B); p65 translocation from cytosol to nucleus in HCEC at 30 and 60 minutes postinfection with C. pseudodiphtheriticum was detected by immunocytochemistry (C). Activation of MAPK pathways in HCEC after incubation with C. pseudodiphtheriticum. At indicated times, cells were harvested and processed for Western blot analysis using antibodies to phospho-p38, phospho-ERK and phospho-JNK and β-actin (D).