Figure 3.

Impact of Ab interaction on Cn capsular size and GXM released. (A) Capsule size measurements of Cn serotype A and D strains were performed for yeasts grown in the absence and presence of Ab. Bars are the averages of the results for 25 cell measurements at each condition, and error bars denote SDs. (B) Representative India ink images displaying the effect of Ab interaction on the capsule size of Cn H99 (serotype A) and B3501 (serotype D). The pictures were taken using a ×100-power field. Scale bar, 2 µm. The experiments were performed thrice, and similar results were obtained. (C) GXM concentration in the supernatant of Cn serotype A and D strain cultures was determined by capture ELISA. Bars are the averages of the results for 10 strains per serotype, and error bars denote SDs. For A and C, asterisk denote P-value significance (P < 0.05) calculated by Student's t-test. Each experiment was performed thrice with similar results obtained.