Fig 5. Experiment 1 and 3: Effects on bacterial colonization of eukaryotic “hub” microbes overlap with effects of location and sampling time.

Each bubble represents the amount of microbial community variation between samples at Tübingen wild sites (Experiment 1) that could be correlated to the factor’s location and sampling time (blue), Albugo abundance (green), and Dioszegia abundance (red) using constrained ordination analysis. About 40% of observed variation in both epiphytic or endophytic bacterial colonization could be attributed to the external factors location and sampling time and about 50% when considering Albugo and Dioszegia in addition (total model). For epiphytes and endophytes, respectively, about 20%–35% and 15%–20% of variation (the overlap percent) linked to location and sampling time could also be correlated to either Albugo sp. or Dioszegia sp. (The “overlap percent” is the “factor overlap” divided by location/sampling time-correlated variation, where “factor overlap” is the percent of total community variation shared by Albugo/Dioszegia and location/sampling time). Black lines show the percent variation correlated to pairs of factors, and stars indicate that the two factors connected by the black line were significantly (p < 0.05) independent of one another.