Fig. S2.

The actin cytoskeleton and tonoplast are in close proximity. (A and B) Actin filaments, marked by GFP-ABD2 (A) are in close proximity to the FM4-64 (red)-stained vacuolar membrane (tonoplast) compared with the microtubule marker MAP4-GFP (B). (C) Stretches of colocalization with the tonoplast are significantly longer for GFP-ABD2 than for MAP4-GFP. ***P < 0.001. (D and E) Actin filaments marked by Lifeact–Venus (D) and FM4-64–stained tonoplast (E). (F) Arrowheads indicate potential contact sites of actin filaments at the tonoplast. Data represent means ± SEM (n = 25 meristematic cells for C). (Scale bars: 5 µm.)