Fig. S7.

Effect of actin interference on auxin-induced SNARE stabilization. (A–C) Seedlings were treated with DMSO (control) (A), LatB (500 nM, 6 h) (B), and JASP (2.5 µM, 6 h) (C). (D–F) The auxin-induced (NAA; 500 nM, 6 h) increase in SNARE abundance (D) was not affected by cotreatment with LatB (E) or JASP (F). YFP-VAMP711 (orange) and PI (green) were used to highlight the vacuole and the cell wall, respectively (G) Data represent means ± SEM. *P < 0.05, ***P < 0.001. (n = 30 cells from six individual seedlings for G). Light gray bar indicates statistical evaluation of NAA treatment compared with NAA and LatB or NAA and JASP cotreatment. ns = not significant. (Scale bars: 5 µm.)