Fig. 7.
Effect of CsA on luciferase activity driven from the endogenous Cox2 gene in LPS-treated mice. LPS (10 mg/kg; 4 h; i.p.) increased COX-2 expression in renal medulla and spleen, visualized using bioluminescent imaging of tissue from Cox2fLuc/+ mice (A). Quantification of imaging data indicated no effect of CsA treatment (4 d; 20 mg/kg per day; s.c.) on LPS-induced COX-2 expression in either the renal medulla (B) or spleen (C). This result was confirmed by qPCR analysis of renal medulla (D) and spleen (E) from WT mice. Data are means ± SEM; n = 3–6. *For all panels, effect of CsA, P > 0.05 by unpaired t test.