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TLRs played an important role in HMGB1-mediated myocardial inflammation. Wt and RAGE-ko mice were treated with AAV9 vectors (HMGB1 or Luc) and immunized only with CFA. Measurements were done on day 21. (A) Myocardial gene expression. (B and C) Protein levels of TLR-2 and -4. Signals from Western blots were normalized against GAPDH. Error bars indicate mean ± SEM. *P < 0.05, **P < 0.01.
