Fig. S3.

Pkd2l1-Cre mouse strain. (A) Map of the Pkd2l1-cre plasmid used to generate the transgenic mouse strain. (B) A Venn diagram of count data comparing PKD2L1 immunoreactivity (red circle) to Pkd2l1-Cre tomato reporter positive cells (yellow circle). Overlap shown in orange. Cre reporter efficiency, as determined by the tomato reporter fluorescence, is ∼79% in the circumvallate taste tissue. (C) Confocal image z stacks of circumvallate taste buds from a triple-transgenic Pkd2l1-Cre, Rosa^Tdt, Trpm5-GFP mouse. Tomato reporter expression is displayed in magenta, and Trpm5-driven GFP fluorescence is displayed in green. Tomato reporter fluorescence and Trpm5-driven GFP fluorescence do not coincide. (All scale bars: 30 µm.)