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Membrane targeting of G–GECO1–Orai1. (A and B) Transmitted light image (A) and single confocal optical section (B) of a resting human T-cell transfected with G–GECO1–Orai1 and human STIM1 5 min after treatment with 1 µM ionomycin. (C) Profile plot of the green channel fluorescence intensity as a function of distance along the red line in B. (Scale bar, 5 µm.)
