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Spatial distribution of high-CAD G–GECO1.2–Orai1 transients. G–GECO1.2–Orai1 Y80E fluorescent spots were identified after shift from 0.1 to 2 Ca and their cumulative positions plotted over 2.5-s (A), 10-s (B), and 40-s (C) intervals for a representative HEK 293A cell. Note that the spots are distributed across the basal surface of the cell in A and progressively fill the basal surface over time (B and C). (Scale bar, 10 µm, for A–C.)
