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. 2015 Dec 30;113(2):338–343. doi: 10.1073/pnas.1523918113

Fig. 4.

Fig. 4.

Double-guide knockdown of Myh6 results in cardiac failure and hypertrophy. (A) Animals that received AAV9-sgRNA against both Myh6 exon 3 and exon 8 displayed a robust decrease in fractional shortening within 3 wk of AAV delivery. ***P < 0.001; n = 18 control animals, 13 edited animals. (B) Four weeks after AAV delivery of dual sgRNAs, animals showed decreased expression of Myh6 by qPCR. *P < 0.05; n = 7 control animals, 6 edited animals. (C) After Myh6 knockdown with two AAV-sgRNAs, animals showed elevated levels of Myh7, Nppa, and Nppb by qPCR within 4 wk of AAV delivery. *P < 0.05, **P < 0.01, ***P < 0.001; n = 7 control animals, 6 edited animals. (D) Animals that were Cas9+ and received dual AAV-sgRNA against Myh6 exhibited cardiac dilation within 4 wk of AAV delivery.