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. 2016 Jan 20;11(1):e0147044. doi: 10.1371/journal.pone.0147044

Fig 2. The quantitative proteomic strategy for fiber marker discovery and fiber proteome profiling results.

Fig 2

(A) The schematic workflow of marker discovery and validation with combined untargeted and targeted proteomic strategies. Two replicates of a specific fiber sample (S1 and S2) were labeled with intermediate and heavy dimethyl tags, a fiber mixture from three species with a light dimethyl label served as reference (REF). The mixture of S1, S2 and REF was analyzed by nanoLC-MS/MS in IDA mode for fiber proteome profiling and marker discovery. Then peptide markers were validated and used for fiber quantification with the parallel reaction monitoring (PRM) approach. (B) Numbers of protein identification in cashmere, wool and yak fiber samples. The result shows large overlap of the fiber proteome among three species. (C) Percentage of keratin and KAPs identified in three types of fibers. Detailed GO classification for each identified protein is shown in S1 Table.