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. Author manuscript; available in PMC: 2016 Jan 20.
Published in final edited form as: Cell Rep. 2015 Dec 10;13(11):2553–2564. doi: 10.1016/j.celrep.2015.11.043

Figure 3. C9 and IM-CKV063 span two neighboring E2 molecules in one spike.

Figure 3

(A) CryoEM reconstruction of CHIKV (S27) virus like particle (VLP) (right), VLP in complex with C9 Fab fragments (left) and VLP in complex with IM-CKV063 fragments (middle). (B) Fitting of E1-E2 and Fab crystal structures into the viral spike at the q3-fold axis of the cryoEM density map of C9 Fab bound VLP (left) and IM-CKV063 Fab bound VLP (right). Blue, Fab; Pink, E2; Cyan, E1. (C) C9 Fab (left) and IM-CKV063 Fab (right) insert between domain A of one E2 (left) and domain B of a neighboring E2 (right) in one viral spike. E2-W64 was labeled in black and E2-G95 was labeled in gray. Comparison of C9 Fab (blue) and IM-CKV063 Fab (hot pink) binding to a viral spike in top view (D) and side view (E).