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. Author manuscript; available in PMC: 2016 Mar 3.
Published in final edited form as: Nature. 2015 Aug 10;525(7567):109–113. doi: 10.1038/nature14670

Extended Data Figure 1. Measurement Of Endogenous And Exogenous Gene Expression In Danio Rerio.

Extended Data Figure 1

(A) Corresponding representative embryos of each morpholino knockdown on the left, with closeup of heart on the right. (B) To assess efficiency of morpholino knockdown, embryos were collected 72 hours after injection, mRNA was collected, and qPCR was performed. We demonstrate that morpholino (MO) knockdown of each indicated gene results in reduced mRNA expression, after normalization to beta-actin expression, compared to mock-injected controls (two-sided Student’s t-test). (C) Western blotting of embryos injected with DCHS1 mRNA demonstrates the production of protein. Mutant mRNA refers to the compound mutant P197L/R2513H. p-values are noted on graphs.