Skip to main content
. 2016 Jan 20;11(1):e0144215. doi: 10.1371/journal.pone.0144215

Fig 1. Specific and efficient endothelial-specific deletion of myd88 in CNS endothelial cells.

Fig 1

A transgene driving the expression of cre recombinase under the control of the tek promoter was used to target endothelial cells. A: Histological detection of GFP expression in a specific vascular pattern in hippocampus sections from ROSA26 reporter mice carrying the tek-cre transgene. B: No recombination was evident in ROSA26 single transgenic mice in the absence of the cre transgene. C: Phase contrast image of CNS microvascular fragments purified using a density gradient method. D: qPCR for the unrecombined myd88 allele in hippocampal microvascular DNA from myd88Flox/Flox (No cre), ΔEndoMyD88 and constitutive deletion (ΔMyD88) mice. Note the near-complete deletion of myd88 in microvascular DNA from ΔEndoMyD88 mice (p<0.001, t-test). Sample size, 3 per genotype. Error bars represent standard error of the mean. Scale bars, 50 μm (A, B); 10 μm (C). E: Diagram of genomic primer binding sites for assays used to generate data in D.