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. Author manuscript; available in PMC: 2016 Jan 20.
Published in final edited form as: Nature. 2015 Mar 16;521(7551):217–221. doi: 10.1038/nature14215

Figure 1. Organ/tissue collapse and misalignment in hir mutants.

Figure 1

a, 1, 1’, Lateral view of live wild-type (WT) and hir mutant embryos, anterior to the left. Arrowheads: heart. Brackets: embryo thickness; 2, 2’, Dorsal view, anterior upwards. Arrowheads: mislocated lenses; 3, 3’ Transverse section at the plane shown in 1 and 1’. Neural tubes (black dots) and somites (red dots). b, 1-3 lateral and 1’-3’ dorsal views of live embryos. Arrowheads: blastoderm margin. Epiboly quantified (%) in (c). Error bars ± S.E.M. (**P < 0.01, ***P < 0.001; one-way ANOVA with Dunnett's T3 post hoc. Figure 1 source data). d, Transverse sections at 5th somite level, neural tube (encircled) and somites (blue) by myoD in situ hybridization. e, Time-lapse sequence of dorsal view of WT and hir mutant right eyes. Arrowheads: lens placode; arrows: invaginating retina. Fragmented and detaching lens placode demarcated by dotted lines in 1’ and 2’. Scale bars: 40 μm.