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. Author manuscript; available in PMC: 2016 Jan 20.
Published in final edited form as: Nature. 2015 Mar 16;521(7551):217–221. doi: 10.1038/nature14215

Figure 3. Cell and tissue dynamics in hir mutants.

Figure 3

a, Schematic: hir neural tube collapse is associated with long chain-like arrangements of neuroepithelial cells generated by increased cell slippage and randomized oriented cell division (Extended Data Fig. 4, 5). b, Whole-mount FN immunohistochemistry (IHC) of st.22 embryos, dorsal view, anterior to the top. 1-1”, Cont (control), WT embryos injected with out-of-frame 70kD N-terminal medaka FN1a+1b mRNA (250 pg) (n=20); 2-2”, uninjected hir mutants (n=11); 3-3”, WT embryos injected with N-terminal 70kDa FN1a+1b mRNA (250 pg) (n=39). 1-3, left anterior head of live embryos (asterisks, lens; triangle, forebrain ventricle); 1’-3’, left eye of FN IHC (green), boxed area magnified in 1”-3”; 4, 5, surface view of FN stained neural tube, WT (n=15) and hir (n=14) corresponding to the region in 1 and 2, respectively, boxed area magnified in 4’ and 5’. Arrowheads: FN fibrils/puncta, arrows: FN large deposits. Scale bars, 40 μm in b1, 1’, 4; 5 μm in b1’’, 4’.