Table 1.

Unique beta-lactamase genes identified from gut and oral microbiomes from healthy humans. Gene ID refers to unique identifier in Table S3 and S4, and enzyme names use established nomenclature (24, 32). Percentage amino acid identity to the closest related gene in GenBank is calculated using the global alignment program clustalW (24, 33).

Beta- Lactamase Family	Enzyme name	Gene ID	Genbank ID	Source	% amino acid identity to NCBI
AmpC	AmpC- EcolK12	AX_iG2_08	GQ343010	Aerobic gut isolate	100.0
	AmpC-EC6	PE_iG1_02	GQ343155	Aerobic gut isolate	100.0
	AmpC-EC31	PE_iG2_05	GQ343162	Aerobic gut isolate	100.0
	AmpC-HG1	AX_iG2_21	GQ343018	Aerobic gut isolate	99.7
	AmpC-HG2	CA_iG2_12	GQ343059	Aerobic gut isolate	99.5
	AmpC-HG3	CF_iG2_01	GQ343071	Aerobic gut isolate	90.0
	AmpC-HG4	CF_iG2_06	GQ343073	Aerobic gut isolate	97.4
	AmpC-HG5	CA_iG1_06	GQ343055	Aerobic gut isolate	99.2
TEM	TEM-1b	AX_iG1_01	GQ343004	Aerobic gut isolate	100.0
	TEM-168	PE_iG2_13	GQ343167	Aerobic gut isolate	99.7
	TEM-169	PI_iG2_05	GQ343173	Aerobic gut isolate	99.1
CTX-M	CTX-M-15	AX_iG1_04	GQ343005	Aerobic gut isolate and metagenomic gut sample	100.0
CblA	CblA-1	AX_iG2_02	GQ343019	Aerobic gut isolate and metagenomic gut sample	100.0
	CblA-2	AX_mG2_03	GQ342999	Metagenomic gut sample	99.7
	CblA-3	PE_mG2_02	GQ343154	Metagenomic gut sample	99.0
CfxA	CfxA6	AX_mG2_01	GQ342996	Metagenomic gut sample	87.2
HGA	HGA-1	CA_mG1_02	GQ343038	Metagenomic gut sample	61.4
HGB	HGB-1	AX_mG2_05	GQ343000	Metagenomic gut sample	58.5
HOA	HOA-1	AX_mO1_01	GQ343035	Metagenomic saliva sample	49.5
HGC	HGC-1	CA_mG1_01	GQ343037	Metagenomic gut sample	48.1
	HGC-2	CA_mG1_04	GQ343039	Metagenomic gut sample	51.0
HGD	HGD-1	CA_mG2_04	GQ343044	Metagenomic gut sample	52.9
HGE	HGE-1	AX_mG2_11	GQ343003	Metagenomic gut sample	37.1
HGF	HGF-1	AX_mG2_09	GQ343002	Metagenomic gut sample	43.3
HGG	HGG-1	PE_mG1_01	GQ343153	Metagenomic gut sample	38.8
HGH	HGH-1	PI_mG1_01	GQ343170	Metagenomic gut sample	34.5
HGI	HGI-1	CA_mG2_07	GQ343045	Metagenomic gut sample	42.6