Figure 4. Preserving redox capacity into old age requires the serotonin receptor SER-5.
(a) Survival of wt (dotted lines) or serotonin receptor mutants and serotonin synthesis mutant (bold lines) treated with water (black) or mianserin (blue) on day 1, followed by increasing concentrations of paraquat on day 5. (b) Bar graph shows fold protection as a ratio of survival of mianserin-treated vs. water-treated GPCR mutant animals ((Mia/water)-1). *P<0.05, **P<0.01, ***P<0.001, n.s., not significant, t-test; Error bars: S.E.M. See Figure 4—figure supplement 1, and Tables 6 and 7 for detailed statistics.
Figure 4—figure supplement 1. This figure relates to Figure 4a in main text.
(a) Survival of wt and two independent alleles of ser-5 mutants, ser-5(tm2647) or ser-5(tm2654), treated with water or mianserin on day 1, followed by increasing concentrations of paraquat on day 5 of adulthood. (b) Hierarchical clustering of fold change [serotonin antagonist/DMSO] in protection of wt (N2) and ser-5 mutant animals, when treated with DMSO or serotonin antagonists on day 1 followed by paraquat on day 5, shows the degree of similarity in protection between 8 structurally different serotonin antagonists (left) and the requirement of ser-5 for these antagonists to protect from oxidative stress. (c) Bar graphs quantifying transcriptional drift by qRT-PCR (log fold-changes in gene expression) in 5-day-old N2 and ser-3(ad1774) animals (left panel), and N2 and ser-4(ok512) animals (right panel) treated with mianserin, relative to water-treated N2, determined by qRT-PCR. Mianserin treatment of ser-3(ad1774) and ser-4(ok512) strains result in a drift pattern, similar to those seen in N2. Thus, these receptors are neither required for drift-attenuation in redox genes, nor for the age-associated increase in oxidative stress resistance (Figure 4). Error bars: S.E.M. For detailed statistics, see Tables 6 and 7.