Table 2.
Survival data for lifespan of RNA-seq experimental cohorts.
| Strain | Treatment | Treatment added on [day] |
Conc. [µM] | Change in lifespan [%] Expt.1/ Expt.2/ Expt.3 |
P-value Expt.1/ Expt.2/ Expt.3 |
Mean Lifespan [days] Expt.1/ Expt.2/ Expt.3 |
Number of animals Expt.1/ Expt.2/ Expt.3 |
|---|---|---|---|---|---|---|---|
| N2 | Water | d1 | 0 | 19.33/ 17.2/ 20.45 | 132/ 149/ 130 | ||
| N2 | Mia | d1 | 2 | +7/ +12/ -4 | 0.20/ 0.04/ 0.25 | 20.64/ 19.23/ 19.67 | 125/ 133/ 151 |
| N2 | Mia | d1 | 10 | +30/ +16/ +6 | 2.5E-07/ 3.7E-03/ 0.55 | 25.09/ 19.92/ 21.74 | 94/ 138/ 136 |
| N2 | Mia | d1 | 50 | +46/ +39/ +25 | 1.1E-19/ 1.9E-15/ 2.8E-08 | 28.25/ 23.92/ 25.63 | 95/ 131/ 125 |
| N2 | Mia | d3 | 50 | +15/ +14/ +1 | 2.0E-03/ 9.3E-04/ 0.29 | 22.23/ 19.69/ 20.75 | 121/ 134/ 152 |
| N2 | Mia | d5 | 50 | -8/ +8/ -2 | 0.18/ 0.06/ 0.84 | 17.79/ 18.52/ 20.13 | 123/ 151/ 139 |
Summary of all lifespan experiments performed in parallel for cohorts 1 and 2 of the RNA-seq studies in Figure 2c,e. The treatments, water or mianserin, at the indicated concentrations (conc.) were added on indicated day (D) of adulthood and lifespan (days) was scored until 95% of animals were dead in all tested conditions. All values (Change in lifespan [%], P-values) were calculated for the pairwise comparison between mianserin-treated and water-treated animals of the same condition, in 3 independent experiments (expts.). Statistical analysis was performed using the Mantel–Haenszel version of the log-rank test. Mean lifespan [days] and number of animals in each experiment are indicated.