Table 3.

Gene ontology (GO) pathways of relevance to this study that are differentially regulated by mianserin.

DOI: http://dx.doi.org/10.7554/eLife.08833.023

KEGG / GO ID	KEGG / GO Term	Number of Genes observed	Levene’s test for variance (Difference in transcriptional drift- variance) Water D1 vs. water Dx	Levene’s test for variance (Difference in transcriptional drift- variance) water Dy vs. mianserin Dy
Transcriptome		19,196	D3 : P < 1.0E-100 D5 : P < 1.0E-100 D10: P < 1.0E-100	D3 : P < 1.0E-100 D5 : P < 1.0E-100 D10: P < 1.0E-100
KEGG:Cel00030	Pentose phosphate pathway	17	D3 : P = 0.0096 D10: P <1.0E-5	D3 : P <1.0E-4 D10: P = 0.01
GO: 0006979	Response to oxidative stress	67	D3 : P <1.0E-10 D10: P <1.0E-16	D3 : P <1.0E-4 D10: P = 0.001
GO: 0045454	Cell redox homeostasis	52	D3 : P <1.0E-6 D10: P <1.0E-10	D3 : P <1.0E-4 D10: P = 0.029
GO: 006749	Glutathione metabolism	13	D3 : P <1.0E-4 D10: P <1.0E-7	D3 : P =0.041 D10: P <1.0E-4
GO: 0007186	G-protein coupled receptor signaling	335	D3 : P <1.0E-24 D10: P < 1.0E-100	D3 : P <1.0E-4 D10: P <1.0E-4
GO: 0016209	Antioxidant activity	34	D3 : P <1.0E-8 D10: P <1.0E-10	D3 : P = 0.002 D10: P = 0.06

Summary of gene changes with RNA-seq transcriptome analysis in Figure 3b.

GO ID is the Gene Ontology identification number.

GO Term is the Gene Ontology term for the biological process.

D_x = age in days for the animals indicated, compared with D1 water-treated animals.

D_y = age in days for water- and mianserin-treated animals, compared on the same day of age indicated.