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. Author manuscript; available in PMC: 2016 Jan 20.
Published in final edited form as: Science. 2014 Nov 13;346(6215):1373–1377. doi: 10.1126/science.1259037

Figure 2. Mutations of the TAL1 enhancer activate through recruitment of MYB.

Figure 2

(A) All TAL1 enhancer mutations introduce de novo MYB binding sites as determined by UniPROBE (19). The MYB primary binding motif is shown above the mutation-derived MYB motifs, with inserted nucleotides shown in red. (B) A 400 bp fragment of the −7.5 kb TAL1 enhancer containing either the wild-type sequence or each of the mutant alleles was cloned upstream of luciferase and a minimal promoter. Constructs were nucleofected into Jurkat cells, together with either control siRNA, or two independent siRNAs targeting MYB. Firefly luciferase activity was measured at 24 hrs, normalized to renilla luciferase to control for cell number and transfection efficiency, and expressed as a ratio relative to activity of the wild-type STIL-TAL enhancer construct. Error bars are ±SEM from two independent experiments performed in triplicate. Corresponding immunoblots for MYB and tubulin are shown below.