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. 2016 Jan 21;4:4. doi: 10.1186/s40635-016-0075-4

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© Kaynar et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Insulin signaling pathway genes expression in Drosophila melanogaster after septic injury with Staphylococcus aureus. a We determined insulin pathway gene (InR, IRS, PTEN, Akt1, Foxo, and mTOR) expression at 6, 18, 48, and 168 h (1 week) after needle pricking. By 18 h, InR was upregulated and significantly higher in infected without treatment group compared to sham and Akt was upregulated with significant difference between all three groups. By 48 h in infected with antibiotics and sham groups, all insulin pathway genes showed sustained elevation of expression without significant difference between groups. In infected with antibiotics group 1 week after infection, levels of InR, IRS, Akt, and mTORC1 were upregulated and significantly higher than those in the sham group. (*p < 0.05). b We determined phosphorylated-Akt/total-Akt ratio with Western immunoblot at 6, 18, 48, and 168 h after needle pricking. Akt phosphorylation significantly decreased by 18 h in infected without treatment and infected with antibiotics compared to sham (asterisk). Akt phosphorylation significantly increased in sham between 18 and 48 h (dollar sign), yet then it was significantly lower at 168 h compared to 48 h (percent). Akt phosphorylation at 168 h after infection was not significantly different compared to 18 h after infection. Akt phosphorylation significantly increased in infected with antibiotics group 48 h after needle pricking compared to 18 h (number sign), yet then it was significantly lower at 168 h compared to 48 h (ampersand). Akt phosphorylation significantly increased in infected with antibiotics group 168 h after needle pricking compared to 18 h after infection (commercial at sign)