Fig. 4.

RT-PCR and Western blot analysis of gelsolin expression in chicken embryos and organs from chicken embryos at stage HH36. a Horizontal DNA electrophoresis of PCR products. As templates for PCR served cDNAs obtained from chicken embryos at different developmental stages (HH9-25) and tissues removed from chicken embryo at HH36 stage. PCRs were carried out using primers spanning exons recognizing GSN mRNA. ACTB, gene coding β actin served as a reference gene. Note different expression levels of GSN and ACTB genes in tested tissues. We observed highest amount of GSN product in skin, eye, meninges, heart, and lung. b Western blot analysis of protein extracts from chicken embryos at different developmental stages (HH9–25) and tissues removed from chicken embryo at HH36 stage for presence of gelsolin at protein level. High amount of gelsolin was in the eye, meninges, gizzard and small intestine; moderate amount of gelsolin was observed for skin, heart, spleen, skeletal muscles, and mesonephros and the lowest amount of gelsolin was detected in brain, lung, liver, and metanephros. As reference β tubulin and β actin were taken. 30 μg of protein was loaded on every line