Table 2.

List of clinical and environmental biofilm samples subjected to cloning of PCR products.

Sample	C/Ea	Allele patternb	ST	Implication
191712	C	6,10,1/15,13,X,14,6	578c	Patient
192147	C	6,10,1,13,2,X,6	–	Patient
163655	C	6,3,9,10,9,1,6	B	Patient
192091	C	X,3,9,10,2,1,6	23c	Patient
cal12_18	E	+,+,+,+,+,+,+	+	Hotel room with 2 cases (1 death)
cal12_40	E	6,10,15,X,9,14,6	578c	Hotel room with 1 case (1 death)
cal12_46	E	6,10,15,13,9,14,6	578	Hotel room with 2 cases (1 death)
cal12_97	E	6,10,+,+,9,14,+	578c	Hotel room with 1 case
cal12_151	E	6,10,15,13,9,14,6	578	Hotel room with 2 cases (2 deaths)
F3	E	2/6,3,9,10,2,1,6	23	Air filter

Underlined bold regions were used (fliC, pilE, asd, mip, mompS, proA, neuA). Selected biofilm samples were taken from different rooms.

^aC, clinical; E, environmental.

^bX, PCR amplification failed; +, positive amplification but allele assignation failed.

^cIncomplete allele pattern but compatible with ST23 or ST578.