Table 3.
Results of the molecular investigation of environmental samples.
| Date | Sample | Location | N | Type of sample | fliC | pilE | asd | mip | mompS | proA | neuA | ST/Species | Serogroup/MAb type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Jan-March 2012 | Several | Hotel rooms, hall, restrooms | 5 | Biofilm | 6 | 10 | 15 | 13 | 9 | 14 | 6 | 578 | – |
| Jan-March 2012 | Several | Hotel rooms, hall, restrooms | 26 | Biofilm | (6) | (10) | (15) | (13) | (9) | (14) | (6) | (578) (at least 5 loci) | – |
| Jan-March | Several | Hotel rooms, hall, restrooms | 74 | Biofilm | + | + | + | + | + | + | + | Incomplete STs (< 5 loci) | – |
| 07/07/2012 | PF2 | Air filter | 1 | Biofilm | 6 | 3 | – | – | – | – | – | – | – |
| 07/07/2012 | PF3 | Air filter | 1 | Biofilm | – | 3 | 9 | – | – | 1 | 6 | – | – |
| 07/07/2012 | F1 | Air filter | 1 | Biofilm | 6 | 3 | 9 | 10 | 2 | 1 | 6 | 1164 | – |
| 07/07/2012 | F2 | Air filter | 1 | Biofilm | 6 | 3 | 9 | 10 | 2 | 1 | 6 | 1164 | – |
| 07/07/2012 | F3 | Air filter | 1 | Biofilm | 2 | 3 | 9 | 10 | 2 | 1 | 6 | 23 | – |
| 31/01/2012 | 918 | Spa pool | 2 | Culture | 6 | 10 | 15 | 10 | 21 | 40 | 6 | 1236 | 1 |
| 11/02/2012 | 12392 | Spa pool | 1 | Culture | 2 | 3 | 9 | 10 | 2 | 1 | 6 | 23 | 1 |
| 23/02/2012 | 2084 | Spa pool | 1 | Culture | – | – | – | – | – | – | – | L. micdadei | – |
| 02/05/2012 | 4855 | Spa pool | 9 | Culture | – | – | – | – | – | – | – | L. micdadei | – |
| 02/05/2012 | 4856 | Spa pool | 12 | Culture | 2 | 3 | 9 | 10 | 2 | 1 | 6 | 23 | 1 |
| 26/06/2012 | 8298 | Room, cold water | 9 | Culture | – | – | – | – | – | – | – | – | 2–15 |
| 04/07/2012 | Al657 | 90 m3 water tank | 10 | Culture | 2 | 3 | 9 | 10 | 2 | 1 | 6 | 23 | 1 |
| 09/07/2012 | 8965 | Osmosis (in) | 7 | Culture | 2 | 3 | 9 | 10 | 2 | 1 | 6 | 23 | 1 |
| 09/07/2012 | 8966 | Osmosis (out) | 5 | Culture | 2 | 3 | 9 | 10 | 2 | 1 | 6 | 23 | 1 |
| 09/07/2012 | 8968 | Spa (fountain) | 10 | Culture | 2 | 3 | 9 | 10 | 2 | 1 | 6 | 23 | 1 |
| 18/07/2012 | 69135 | Spa pool | 1 | Culture | – | – | – | – | – | – | – | L. micdadei | – |
| 18/07/2012 | 69147 | Spa pool | 1 | Culture | – | – | – | – | – | – | – | L. micdadei | – |
| 18/07/2012 | 69148 | Osmosis device | 1 | Culture | 2 | 3 | 9 | 10 | 2 | 1 | 6 | 23 | 1 Allentown/France |
| 18/07/2012 | 69153 | Spa pool | 1 | Culture | 2 | 3 | 9 | 10 | 2 | 1 | 6 | 23 | 1 Allentown/France |
| 18/07/2012 | 69183 | Spa pool drainer | 1 | Culture | 2 | 3 | 9 | 10 | 2 | 1 | 6 | 23 | 1 Allentown/France |
| 27/07/2012 | Al763 | Watering network (hotel entrance) | 1 | Culture | 5 | 2 | 22 | 10 | 6 | 25 | 203 | 1358 | 8 |
| 23/08/2012 | 10636 | Water deposit | 1 | Culture | – | – | – | – | – | – | – | L. anisa | |
| 12/04/2012 | 4029 | Spa pool | 4 | Culture | 2 | 3 | 9 | 10 | 2 | 1 | 6 | 23 | 1 |
| 12/04/2012 | 4029 | Spa pool | 1 | Culture | 6 | 10 | 15 | 10 | 21 | 40 | 6 | 1236 | 1 |
| 12/04/2012 | 4029 | Spa pool | 1 | Culture | – | – | – | – | – | – | – | L. micdadei | – |
| 02/05/2012 | 4857 | Spa pool | 3 | Culture | 2 | 3 | 9 | 10 | 2 | 1 | 6 | 23 | 1 Allentown/France |
| 02/05/2012 | 4857 | Spa pool | 1 | Culture | 6 | 10 | 15 | 10 | 21 | 40 | 6 | 1236 | 1 Allentown/France |
| 02/05/2012 | 4857 | Spa pool | 1 | Culture | 5 | 2 | 22 | 10 | 6 | 25 | 203 | 1358 | 10 |
| 02/05/2012 | 4857 | Spa pool | 2 | Culture | – | – | – | – | – | – | – | L. micdadei | – |
| 31/01/2012 | Al47 | Spa pool | 3 | Culture | 6 | 10 | 15 | 10 | 21 | 40 | 6 | 1236 | 1 |
| 31/01/2012 | Al47 | Spa pool | 3 | Culture | – | – | – | – | – | – | – | L. micdadei | – |
Biofilm samples from hotel rooms, hall and restrooms (n = 164) were analyzed directly as none produced positive cultures. The results of those samples in which L. pneumophila was detected (n = 105) are shown in the first three rows depending on whether they yielded a complete ST, an almost complete ST (at least five alleles determined to be identical to those defining the ST), or an incomplete ST (less than five alleles determined). For some cultures, several colonies were sequenced, as detailed (N when type of sample is “Culture”). The last three cultured samples revealed a complex composition with two or more STs and/or another Legionella species.