Figure 5.

MiR-324-5p regulates mitochondrial fission and apoptosis through Mtfr1. (a and b) MiR-324-5p suppresses the expression of Mtfr1, mitochondrial fission and apoptosis in the presence of Mtfr1 with wild-type 3′UTR rather than mutated 3′UTR. Cardiomyocytes were infected with adenoviral Mtfr1-wt-UTR or Mtfr1-mut-UTR and were transfected with miR-324-5p mimic. After transfection, cells were treated with A/R. Mtfr1 levels were detected by immunoblot (a, upper panel). Mitochondrial fission (a, lower panel) and apoptosis (b) were assayed. *P<0.05. (c and d) The inhibitory effect of miR-324-5p on mitochondrial fission and apoptosis is abolished by Mtfr1 target protector. Cardiomyocytes were co-transfected with miR-324-5p mimic, Mtfr1 target protector (Mtfr1-TP^miR-324-5p) or the protector control (Mtfr1-TP^control), and then were treated with A/R. Mitochondrial fission (c) and apoptosis (d) were detected. *P<0.05