Figure 3.

Treatment with cisplatin induced prolonged activation of pSTAT1-Ser727. (a) STAT1 expression in the organ of Corti (OC), stria vascularis (SV), modiolus (M), and brain (B) from STAT1 wild-type (+/+) and knockout (−/−) mice. Phosphorylated and non-phosphorylated STAT1 were detected in wild-type tissues. SV exhibited a higher STAT1 content than M and OC. Two western blots were performed, each of them with a pool of six explants for each genotype. Brain was originally used as the control tissue. The asterisks indicate nonspecific bands. (b, c) Explants were treated with 160 μM cisplatin or 50 μM gentamicin for 6 h (b) and 24 h (c). Note that pSTAT1-Tyr701 was barely detectable in wild-type tissue. STAT1 expression was induced by cisplatin, but not by gentamicin. GAPDH was used as a loading control. The data are representative of two independent experiments. Six explants were pooled for each genotype and condition