Figure 4.

Cisplatin decreased STAT3 expression, and gentamicin induced activation of STAT3. (a) STAT3 expression in the organ of Corti (OC), stria vascularis (SV), modiolus (M), and brain (B) from STAT1 wild-type (+/+) and knockout (−/−) mice. No change of STAT3 expression was observed in OC, SV, M, or B from STAT1 wild-type (+/+) or knockout (−/−) mice. Modioli from STAT1^−/− mice exhibited higher STAT3 content. Two western blots were performed, each of them with a pool of six explants for each genotype. Brain was originally used as the control tissue. (b, c) Explants were treated with 160 μM cisplatin or 50 μM gentamicin for 6 h (b) and 24 h (c). STAT3 expression and phosphorylation was inhibited by cisplatin, but the expression of pSTAT3-Tyr705 was induced by gentamicin. GAPDH was used as a loading control. The data are representative of two independent experiments. Six explants were pooled for each genotype and condition