Skip to main content
. 2015 Dec 17;6(12):e2019. doi: 10.1038/cddis.2015.362

Figure 7.

Figure 7

Prolonged activation of c-Jun by cisplatin. Explants from STAT1+/+ and STAT1−/− mice were treated with 160 μM cisplatin or 50 μM gentamicin for 6 h and 24 h (a) and subjected to western blotting. Cisplatin induced the sustained expression of c-Jun for at least 24 h. Gentamicin induced early activation of c-Jun, but less pronounced than cisplatin treatment. Because the expression of total c-Jun protein changes, p-c-Jun was normalized against GAPDH, which was used as a loading control. Densitometric analysis of each protein normalized against WT control (untreated) is shown. The data are representative of two independent experiments. Six explants were pooled for each genotype and condition. (b, c) Quantification of hair cell survival in explants of STAT1−/− mice. Explants were treated with 160 μM cisplatin (b) or 50 μM gentamicin (c) in the presence of SP600125 for 24 h. n=4–5 explants of each genotype and each condition. Values shown are mean+S.D.