Figure 5.

TonEBP exhibited anti-apoptotic and anti-oxidative effects in HCMs under ischemia. TonEBP overexpression significantly increased the ratio of cell viability (a, N=3), decreased cell injury (b, N=3) and attenuated DNA fragmentation (c, N=3) in HCMs under the condition of OGD. Similarly, the TonEBP overexpression could attenuate the increased ROS (d, N=3) and calcium content (e, N=3) and ameliorate the depletion of ATP content (f, N=3) in OGD HCMs. Also, TonEBP overexpression could significantly reduce caspase-3 activity (g, N=3), inhibit the release of cytochrome c (h and i, N=3) and decrease the ratio of Bax/Bcl-2 (h, j, N=6) in HCMs under ischemic insults. Staining for RFL-32 was performed to indicate approximate equal loading of samples. Staining for COX IV was performed to indicate approximate equal loading of mitochondrial samples. (Data were expressed as mean±S.E.M. *P<0.05; **P<0.01; NS, no significance; OE, overexpression; OGD, oxygen glucose deprivation; Tau, taurine; Vec, vector)