Figure 4.

Tnfsf14 regulates myoblast differentiation through Akt. (a) Primary mouse myoblasts (n=4) and C2C12 cells (n=5) were infected with shRNA lentiviruses and differentiated for 24 h, followed by cell lysis and western analysis. (b) C2C12 cells were infected with shRNA lentiviruses as indicated and differentiated for 24 or 72 h, followed by cell lysis and western analysis (n=5). (c and d) C2C12 cells were infected with shRNA lentiviruses as indicated, and then transfected with a constitutively active Akt or a control (pcDNA) construct, followed by differentiation for 72 h and subsequent staining for MHC and with DAPI. Fusion index and total nuclei number were quantified (n=4). One-sample two-tailed t-test was performed for data in (b) and paired two-tailed t-tests for data in (c) and (d). *P<0.05; **P<0.01. All error bars represent S.D. of independent replicates