Figure 5.

Tnfsf14 is required for robust muscle regeneration in vivo. (a) TA muscles were injured by BaCl₂ injection, and isolated on days 3, 5, 7 and 14 AI. Upon cryosection, H&E staining or immunofluorescence staining for Tnfsf14, HVEM and LTβR (green) together with DAPI (blue) were performed (n=3). (b) TA muscles were co-injected with BaCl₂ and shRNA viruses, and then processed as described in (a). (c) Quantification of the number of regenerating myofibers on muscle sections from (b). (d) Quantification of the regenerating myofiber cross-sectional area (CSA) on muscle sections from (b). For each time point in (b–d), five or six mice were analyzed. All error bars represent S.D. of independent replicates. Paired two-tailed t-test was performed to compare shScramble and shTnfsf14 at each time point. **P<0.01. Scale bars: 50 μm