Figure 3. Mmb1Δ cells have mitochondria positioning defects.
(A) Images of wildtype and mmb1Δ cells expressing cox4-GFP. Mmb1Δ cells show mitochondria positioning defects. Bar, 5 μm. (See also Movies S1A, S1B and S2A, S2B)
(B) Quantification of mmb1Δ mitochondria aggregation phenotypes. 1 = wildtype-like, 2 = aggregation at one cell end, 3 = aggregation at both cell ends, 4 = aggregation at both cell ends but with minor connection. Plot shows percentage of interphase wildtype and mmb1Δ cells with the different mitochondria aggregation phenotypes. (See also Fig. S3)
(C) Time-lapse images of wildtype and mmb1Δ cells expressing cox4-GFP. Both cell types exhibit apparent mitochondria fission (yellow circles) and fusion (red circles). Bar, 5 μm. (See also Movies S3A and S3B)