Table 1.

Optimization of the concentration of HRP-V in the blank assay: the absorbance values read at 450 nm for both HRP-V and HRP are reported, as well as the ratio between the two sets of data.

	Absorbance at 450 nm
Dilutions	HRP-V	HRP	Absorbance Ratio HRP-V/HRP
1:200	1.804 ± 0.02	1.059 ± 0.18	1.70
1:400	1.670 ± 0.2	0.803 ± 0.10	2.08
1:800	0.878 ± 0.05	0.387 ±0.02	2.26
1:1600	0.572 ± 0.05	0.252 ± 0.01	2.27

Errors in the absorbance measurements represent ± 1 standard deviation and are for experiments performed in triplicate.