Fig. 3.

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis of gene transcripts in the intestines of fed, fasted and refed X. laevis. RNAs were prepared from the intestines of the frogs that were fed for 22 days, fasted for 22 days (fasted), or fasted for 21 days and then refed for 1 day (refed), followed by RT-qPCR (n = 8). Transcripts of 74 genes were divided to the following categories: digestion or absorption (22 genes), apoptosis (7 genes), proliferation (8 genes), regulation of gene expression (19 genes), metabolism (16 genes) and others (2 genes). Color scale indicated fold changes of gene expression. The data of the full name of the genes tested, the exact fold changes, SEM and statistic analysis are shown in Additional file 2: Table S1. These experiments were repeated at least two times, with similar results